Composite

Part:BBa_K3852002:Design

Designed by: Yuan Cao   Group: iGEM21_BIT-China   (2021-09-30)


Pfba1+T2R1+eGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 638
    Illegal XbaI site found at 1345
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 638
    Illegal XbaI site found at 1345
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 638
    Illegal XbaI site found at 1345
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to make the receptor better localize on the cell membrane of S. cerevisiae, the signal peptide sequence of Ste2 was added to its front end.


Source

Derived from the human genome

References